Endocrine Abstracts

Our previous work has shown that the intake of a high fat high carbohydrate (HFHC) meal results in an increase in endotoxin (LPS) and LPS binding protein (LBP) concentrations concomitant with an increase in the expression of TLR-4, the receptor for LPS, and CD-14, which facilitates the binding of LPS to its receptor. In addition, there is an increase in ROS generation, NFkB binding and the expression of TNF-α and IL-1β. Our work has also demonstrated that an equicalo...

We have previously demonstrated that a low dose of hydrocortisone (100 mg) given intravenously suppresses intranuclear NFκB and AP-1 binding and the expression of pro-inflammatory genes like MMPs. We have now investigated the effect of a high dose of hydrocortisone (300 mg=60 mg prednisolone) on NFκB binding and the expression of TLRs, the mediators of TLR signal transduction, MyD88 and TRIF and HMG-B1. Ten normal subjects were injected intravenously with 300 mg of h...